In vitro fibroblast cells culture from Pelung chicken embryo and its potential application

Pock forming ability of fowl pox virus isolated from layer chicken and its adaptation in chicken embryo fibroblast cell culture.

AIM The objective of the present study was to examine pock forming ability of field strain and vaccine strain of fowl pox virus (FPV) in chorioallantoic membrane (CAM) of embryonated chicken eggs and its adaptation in chicken embryo fibroblast (CEF) cell culture. MATERIALS AND METHODS Dry scabs were collected from 25 affected birds in glycerin-saline and preserved at 4°C until processed. Viru...

In Vitro Maturation, Fertilization and Embryo Culture of Oocytes Obtained from Vitrified Auto-Transplanted Mouse Ovary

Chicken Embryo Spinal Cord Slice Culture Protocol

Slice cultures can facilitate the manipulation of embryo development both pharmacologically and through gene manipulations. In this reduced system, potential lethal side effects due to systemic drug applications can be overcome. However, culture conditions must ensure that normal development proceeds within the reduced environment of the slice. We have focused on the development of the spinal c...

The in vitro culture of embryonic chicken heart cells.

IN the early 1900’s, Burrows [l ] and Carrel [2] reported the serial cultivation of cells derived from an adult chicken heart. Since that time, numerous laboratories have experienced difficulties in long-term avian cell culture. After many unsuccessful attempts, with several modifications in technic, embryonic chick heart cells were established and maintained in this laboratory as monolayer cul...

O-8: Clinical Application and Evaluation of Autologous Keratinocyte and Fibroblast Cells Culture on Horse Open Wound Healing

KFGS (Keratinocyte fibrin glue suspension) and FKFGS (Fibroblast keratinocyte fibrin glue suspension) methods were performed in this study. Materials and Methods: Four healthy adult horses were used in this study (350 ± 117). Fibroblast cells have been separated by explant culture method from neck, keratinocyte cells have been separated by enzyme digestion from lib skin samples and Fibrinogen h...